RP—HPLC法测定其木德玛汤中没食子酸的含量

Determination of Gallic acid in Qimudema Soup by RP-HPLC

目的：测定其木德玛汤中没食子酸的含量。方法：没食子酸含量分析采用高效液相色谱法。色谱条件：流动相为乙腈-含0．1％5-乙胺的0．1％磷酸溶液（1：99），检测波长273nm，流速1．0ml／min。结果：样品中没食子酸达到基线分离；没食子酸在0．0605-0．2117μg范围内呈良好的线性关系（r=0．9998）。平均加样回收率为98．98％（1LSD-0．56％）。结论：所建立的含量测定方法简便、准确、具有实用性。

Objective： To develop a method for determination of gallic acid in Qimudema soup. Methods： Gallic acid was analyzed on an Apollo C18 column and the mobile phase consisted of acetonitrile, 0.1% phosphate acid solution containing 0.1% and triethylamine （ 1：99 ）. The detection wavelength was 273 nm and the flow rate of 1.0ml/min. Results： The linearity was good over the range of 0.0605-0.2117 μ g （r =0.9998 ）. The average recovery was 98.98% （n=9）. Conclusion： The established method is simple, accurate and suitable for the determination of gallic acid in Qimudema soup.