摘要
目的探讨用猪小肠黏膜下层(SIS)作支架,复合胰岛体外构建组织工程胰腺的可行性。方法用改良方法分离纯化胰岛并培养,再与SIS进行体外复合培养,分别进行相差显微镜、透射电镜、扫描电镜检查。观察胰岛在SIS上的生长、细胞分泌及细胞活性情况。结果胰岛在SIS材料上粘附、生长,细胞功能活跃,在SIS上成复层生长,复合培养时间明显延长。结论SIS与胰岛在体外复合培养可以构建出类似生物膜的组织工程胰腺,为进一步研究体内胰岛移植奠定基础。
Objective To investigate the feasibility of artificial pancreas construction with islets in a tissue scaffold of small intestinal submucosa(SIS).Methods Pancreatic islets were isolated and purified using a modified method.The islets co-cultivated with SIS were observed by phase contrast microscope,electronic microscope and scanning electronic microscope.Islet growth and secretion function were evaluated.Results The islets adhered to and grew on SIS,with active cell function.Islets grew well in layers,and the survival time was significantly longer.Conclusion Combined culture of SIS and islet in vitro can construct an artificial pancreas similar to the biomembrane,thus providing a base for further study of islet transplantation in vivo by tissue engineering technique.
基金
陕西省"13115"科技创新工程重大科技专项项目计划(2007ZDKG-67)
国家自然科学基金(30772096)
卫生部临床学科重点项目(胰岛移植物营养支持及免疫保护的研究)
陕西省自然科学基金项目(2007C212)
西安交大医学院附属第一医院基金青年创新项目(2006YK4)
陕西卫生厅科研基金(08D25)
关键词
胰岛
小肠黏膜下层
组织工程
Pancreatic islet Small intestinal submucosa Tissue engineering