摘要
目的人类白细胞抗原G（HLA-G）作为一种具有免疫抑制功能的分子正日益受到关注,本研究检测了肾移植患者序列血清标本中可溶性HLA-G（sHLA-G）的表达,以阐明其在肾移植中的临床意义。方法用ELISA方法检测65例患者总计330份血清标本的sHLA-G。在此之前,已进行HLA-IgG/IgM抗体和MICA抗体的检测。结果通过对65例患者的330份血清标本的系列分析,在肾功能正常组的26例患者中,50﹪的患者sHLA-G持续表达或转为阳性,相比较而言,移植肾排斥组的39例患者中仅20.5﹪的患者sHLA-G持续表达或转为阳性（P=0.013）。因此,sHLA-G与移植肾功能的维持有关联。80﹪的HLA-IgG阳性的血清中没有sHLA-G表达（77/96）,而81.4﹪的HLA-G阳性的血清中没有HLA-IgG（83/102）（P=0.005）,表明sHLA-G的表达和HLA-IgG抗体（先前已被证明与移植肾失功相关）的出现呈负相关。结论在本研究中,发现约30﹪的肾移植患者血清中存在sHLA-G;sHLA-G与移植肾慢性排斥失功呈负相关,也与HLA-IgG抗体的产生呈负相关。sHLA-G在肾移植中的确切意义有待于进一步研究。
Objectives HLA-G is a potentially interesting molecule associated with immunosuppressive function. Here we try to define it＇s functions in renal transplants. Methods We survey the presence of soluble HLA-G （sHLA-G） in serial serum samples of renal transplants with ELISA tests. Results After serial analysis of the 65 patients＇ 330 sera,50﹪ of 26 patients in functioning group had consistent sHLA-G expression or became positive,in comparison to 20.5﹪ among 39 patients who rejected their transplants（ P = 0.013）. Thus sHLA-G was associated with functioning transplants. Eighty percent （77 of 96） of the HLA IgG positive sera had no sHLA-G expression,while 81.4﹪ （83 of 102） of the HLA-G（＋） sera had no HLA IgG （ P = 0.005）,which showed a negative association between sHLA-G and the presence of HLA IgG antibodies （which was previously been shown to be associated with failure）. Conclusions In this preliminary survey,sHLA-G was found in the serum of about 30﹪ of renal transplant patients. sHLA-G had a negative association with allograft failure from chronic rejection,and a negative relationship with the production of HLA IgG antibodies. The significance of sHLA-G in renal transplants remains to be determined.
