抑制性核酸序列对BXSB狼疮小鼠免疫状况的影响

Influence of the inhibitory oligonucleotides on the immune status of BXSB lupus mice

目的 研究抑制性核酸序列是否可改善BXSB狼疮小鼠的免疫状况,为治疗系统性红斑狼疮提供新的策略.方法 3月龄雄性BXSB小鼠分为三组,包括抑制性核酸序列治疗组、0.9%氯化钠注射液组和空白对照组,治疗前后测定24 h尿蛋白;治疗后检测血清抗dsDNA抗体、肾脏免疫复合物以及外周血淋巴细胞凋亡情况.结果 各组小鼠治疗前的24 h尿蛋白差异无统计学意义（P〉0.05）;治疗后,抑制性核酸序列治疗组小鼠的尿蛋白和抗dsDNA抗体水平明显低于对照组（P〈0.01）;抑制性核酸序列治疗组小鼠的肾脏免疫复合物优于其他治疗组（P〈0.05）;与对照组相比,抑制性核酸序列治疗组小鼠的外周血凋亡明显增加（P〈0.05）;与0.9%氯化钠注射液组相比,抑制性核酸序列治疗组小鼠的生存期明显延长（P〈0.01）.结论 抑制性核酸序列可显著改善BXSB狼疮小鼠的免疫状况,能延缓BXSB狼疮小鼠的疾病进程.

Objective To research whether the inhibitory oligonucleotides could improve the immune status of the BXSB mice. The purpose was to provide a valuable direction for treating systemic lupus erythematosus. Methods 3-month-old BXSB lupus mice were divided into three groups （including inhibitory oligonucleotides group, saline group and blank control group）. The 24 hours urine proteins were determined before treatment. After treatment,the urine protein, anti-dsDNA, peripheral blood lymphocytes apoptosis and immune complex in renal glomeruli were measured. Results Before treatment,the urine proteins had no statistical differences among the three groups （ P 〉 0.05 ）.After treatment,the urine protein, anti-dsDNA levels in inhibitory oligonucleotides group were significantly lower than that of control group（P 〈 0.01 ）. Apoptosis of peripheral blood lymphocytes in inhibitory oligonucleotides group was significantly higher than that of control group（ P 〈 0.05 ） ,immune complex in renal glomeruli in inhibitory oligonucleotides group was significantly lower than that of the other groups （ P 〈 0.05 ）. Compared with saline group, inhibitory oligonucleotides had prolonged life period of BXSB mice （ P 〈 0.01 ）. Conclusion The inhibitory oligonucleotides could improve immune status of BXSB, and could put off the disease progression.