长托宁对急性全脑缺血再灌注大鼠肺损伤的保护作用

Protective Effects of Penehyclidine Hydrochloride on Lung Injury of Rats After Brain Ischemia Reperfusion

目的:通过观察长托宁对全脑缺血再灌注大鼠肺内一氧化氮合酶(NOS)活性的影响,探讨长托宁的肺保护作用及其机制。方法:按改良的Pulsinelli方法建立全脑缺血再灌注模型,分别观察假手术组(A组)、缺血再灌注组(B组)和长托宁治疗组(C组)各时间点(2、12、24、48h)肺组织病理学改变并测定其组织NOS活性。结果:①A组未见组织病理学改变,B组均出现组织病理学损伤,以12h时最严重,C组明显改善;②B组总NOS活性在2、12、24h比A组高,以12h时升高最显著,其中在2h时以结构型(cNOS)升高为主;在12h时以诱导型(iNOS)升高为主,且24h时仍升高,48h时基本接近A组水平;③与B组相比,C组cNOS活性在2h时上升更为显著,iNOS在12h及24h时明显下降。结论:①在全脑缺血再灌注时的不同阶段存在不同程度肺损伤;②在脑缺血再灌注不同阶段肺组织中不同类型NOS活性不同,发挥不同作用,长托宁可以调节肺组织不同类型NOS活性,对抗肺组织损伤。

Objective：To explore the protective effects of Penehyclidine on lung injury of rats after cerebral ischemia reperfusion based on the activities of NOS.Methods：The cerebral ischemia reperfusion models were established by Pulsinelli＇s.Pathologic changes of the lung tissue and activities of NOS were observed at four different times in sham operation group （group A）,ischemia-reperfusion group （group B） and treatment group （group C）.Results：① There were no pathologic changes in group A,but the changes were obviously observed in group B especially at 12h and were significantly improved in group C.② The activities of total NOS were increased at 2 h,12 h and 24 h in group B compared with those in group A,and they were increased significantly at 12 h.The activities of cNOS and iNOS were increased significantly at 2 h and 12 h respectively.③The activities of cNOS increased much higher at 2 h in group C compared with those in group B,but iNOS were significantly decreased at 12 h and 24 h.Conclusions：Various types NOS play different roles in pulmonary injury after brain ischemia-reperfusion in the different stages in rats.Penehyclidine can improve the damages through modulating the activities of different types of NOS.