隆朋麻醉及其手术下对犬内皮素和降钙素基因相关肽的影响

Effect of Rompun anaesthesia and operation on dogs endothelin and calcitoningene-related peptide secretion

探讨单纯隆朋麻醉和隆朋麻醉下手术对犬内皮素(ET)和降钙素基因相关肽(CGRP)分泌的影响。方法:选取健康犬12只,将其随机均分为单纯隆朋麻醉组和隆朋麻醉手术组。两组均于麻醉前、0.5 h、2 h、8 h、24 h、48 h、72 h和120 h采集静脉血,并迅速离心取血浆,用放射性免疫法检测ET和CGRP的浓度。结果:麻醉组,ET出现上升趋势而CGRP呈现下降趋势。ET到2 h时恢复至麻前水平,CGRP到8 h时恢复至麻前水平。手术组,手术开始后ET和CGRP都呈现上升趋势,两者都在24 h时恢复至正常水平。结论:隆朋单纯麻醉时对ET和CGRP有轻微的影响,但隆朋麻醉手术时ET和CGRP都出现上升趋势,机体的应激反应较强烈。

Objective：To study the effect of ET and CGRP externalization on dogs with simple Rompun anaesthesia and operation with Rompun anaesthesia.Methods：To select and equally divide 12 healthy dogs into Rompun anaesthesia group and operation with Rompun anaesthesia group.The venous blood samples were collected before and after anaesthesia at 0.5,2,8,24,48,72 and 120 h for centrifugation to obtain sera.The ET and CGRP′s concentrations were detected with radioimm umoassay.Results：Rompun anaesthesia group：ET appears to higher tendency and CGRP appears to lower tendency.ET regains to the normal concentration in 2 h and CGRP regains to the normal concentration in 8 h.Operation with Rompun anaesthesia group：ET and CGRP are both in higher tendency after operation.Both ET and CGRP fell to the normal concentration in 24 h.Conclusion：There are slight influence to dogs＇ ET and CGRP when simple Rompun anaesthesia but ET and CGRP′s concentration rise and dogs′ stress reaction is intensive when operation with Rompun anaesthesia.