不同遮荫条件下巫山淫羊藿生长状态及生理变化的研究

Research on growth state and physiological changes of Epimedium wushanense under different shade conditions

目的：通过分析不同光照条件下（100%,80%,60%,40%,20%）巫山淫羊藿的各项生长指标和生理指标,探讨巫山淫羊藿对遮荫环境的生理适应机制,为药用淫羊藿生产栽培提供理论依据。方法：以巫山淫羊藿为研究对象,测定了不同遮荫处理下巫山淫羊藿植株形态指标、光和色素含量、脯氨酸含量、丙二醛含量、可溶性糖含量、可溶性蛋白含量、抗氧化性酶活性。结果：除长宽比差异不显著外,其他形态指标都出现显著性变化,在80%40%光强处理下的巫山淫羊藿生长良好,地上部分生物量相对较高;遮荫后叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量增加,叶绿素a/叶绿素b的值在100%60%光强处理下逐渐增大,60%20%光强处理下逐渐减小;遮荫后脯氨酸、丙二醛含量降低;可溶性糖、可溶性蛋白含量分别在100%60%,100%40%光强处理下逐渐增加,60%20%,40%20%光强处理下逐渐下降;遮荫后SOD,CAT活性值减小;POD,APX活性值在100%40%光强处理下逐渐减小,在20%光强处理下有所增大。结论：80%40%光强处理有利于巫山淫羊藿生长。

Objective： To study the growth state and physiological changes of Epimedium wushanense under different light conditions（100%,80%,60%,40%,20%）,and investigate its adaptation mechanism to shade environment so that it can provide theoretical basis for cultivation and production.Method： The growth index,the content of photosynthetic pigments,proline content,MDA content,soluble sugar content,soluble protein content and antioxidant enzymy activity of E.wushanense under different shade conditions were measured.Result： Except for length/width,the other form index of E.wushanense changed significantly.The E.wushanense grew well between light intensity 80%-40%,besides,the biomass above the earth was relatively high.The chlorophyll a,chlorophyll b,total chlorophyll content and carotenoids cotent were enhanced after shaded,the values of chlorophyll a／chlorophyll b increased between light intensity 100%-60% and reduced between light intensity 60%-20%.The proline and MDA content decreased after shading,the soluble sugar and soluble protein content increased respectively between light intensity 100%-60%,100%-40% and reduced respectively between light intensity 60%-20%,40%-20%.The SOD activity,CAT activity decreased after shading,and the POD activity,APX activity reduced between light intensity 60%-40%,raised under 20% treatment.Conclusion： 80%-40% irradiance treatment was favorable to the growth of E.wushanense.