正交试验法优选水牛角水解工艺

Optimization of extraction process of total amino acid from Bubali Cornu by orthogonal test

目的:优选水牛角中总氨基酸水解提取的最佳工艺。方法:以提取物中总氨基酸的含量及提取物对凝血酶引起纤维蛋白凝固的时间为考察指标,采用正交试验,以酸水浓度、酸水用量及水解时间为因素,优选最佳水解条件。结果:水牛角加8倍量4 mol.L-1硫酸回流加热水解提取6 h为水牛角最佳水解工艺。结论:优选的水解工艺提取的氨基酸含量较高,工艺稳定可行。

Objective： To optimize the extraction conditions of the total amino acid from Bubali Cornu.Method： An orthogonal test of L9（3^4）was designed to select optimum exaction conditions of the total amino acid.The influence of solvent concentration,the dosage of solvent and time of extraction were investigated with the content of total amino acid and thrombin-induced fibrin clotting time as index.Result： The optimum extraction condition procedure was described as follows： the concentration of the solvent was 4 mol·L^-1,volume of solvent was 6 times amount of the materials.Conclusion： The optimum exaction conditions procedure is reasonable and stable.