道地和非道地苍术对类风湿性关节炎大鼠病情及促炎细胞因子和炎症介质的影响

Comparative study of effect of Atractylodes lancea between geo-authentic and non-authentic producing areas on collagen-induced arthritis in rats

目的:研究道地产区江苏茅山苍术对类风湿性关节炎(RA)的作用及机制,并与非道地产区湖北英山和陕西华阴苍术进行比较。方法:建立大鼠胶原诱导性关节炎(CIA)模型,评估发病率及临床积分,观察关节组织病理形态和放射学改变,检测血清抗Ⅱ型胶原抗体IgG和IgM水平、关节组织匀浆上清和血清中促炎细胞因子IL-1β,TNF-α和IL-6以及血清炎症介质PGE2的含量变化。结果:模型组大鼠CIA发病率近88%,关节炎平均临床积分8,关节出现明显的炎症细胞浸润、血管翳形成、软骨和骨破坏等组织病理学变化以及关节间隙狭窄或增宽、关节面虫蚀样破坏等影像学改变,血清IgG,IgM水平增高,关节组织和血清中IL-1β,TNF-α和IL-6以及血清PGE2含量明显升高;与模型组相比,江苏苍术组显著降低关节炎的发病率和临床积分、关节组织病理学积分和X光片的骨破坏程度以及血清IgG和IgM水平,还显著降低关节组织和血清中细胞因子和炎症介质的含量;湖北和陕西苍术组也能较明显抑制血清IgM,湖北苍术组尚能降低血清IL-1β和关节组织中IL-6的含量,但对关节炎的其他抑制作用均不明显。结论:道地产区江苏茅山苍术能有效控制大鼠CIA病情的发生发展,其作用机制可能与抑制促炎细胞因子和炎症介质的产生有关。非道地产区湖北英山和陕西华阴苍术对大鼠CIA没有明显的防治作用。

Objective： To compare the effects of Atractylodes lancea from different producing area on collagen-induced arthritis（CIA） in rats.Method： Wistar rats were induced by type II bovine collagen.CIA rats were treated daily with oral administration of A.lancea from the geo-authentic and non-authentic producing area of Maoshan,Jiangsu province,and non-geo-authentic and non-authentic producing areas of Yingshan,Hubei province and Huayin,Shanxi province from day 7 after the day of the first immunization to day 35.Clinical symptoms as well as clinical scores and incidence were observed.All rats were sacrificed on day 35 after immunization to observe histopathologic and radiologic changes.Antibody to type II collagen in sera was measured by enzyme-linked immunosorbent assay（ELISA）,the levels of pro-inflammatory cytokines tumor necrosis factor α（TNF-α）,interleukin-1β（IL-1β）,and interleukin-6（IL-6） in sera and article-homogenated supernants by radiommunoassay,and inflammatory mediator of PGE2 in sera using ELISA.Result：A.lancea from Jiangsu province can ameliorate clinical symptom,reduce arthritis index and arthropathy of inflammatory joints,inhibit the production of IgG and IgM in sera,directly suppress the production of exogenous and endogenous cytokines of IL-1β,TNF-α and IL-6 and PGE2.A.lancea from Hubei and Shanxi provinces can inhibit the production of IgM in sera,and A.lanceas from Hubei province also depress the production of IL-1β in sera and IL-6 in supernants.Conclusion： A.lancea from Jiangsu province is effective in CIA rats through inhibiting the prodution of pro-inflammatory cytokines and the inflammatory mediators.