怀山药试管块茎形成过程中内源激素的变化

Variation of endogenous hormones in formation of microtuber of Dioscorea opposite in vitro

目的:通过分析5种内源激素GA3,IAA,ABA,ZR和JA在山药试管块茎形成建成中的动态变化,初步探讨山药试管块茎形成的生理生化机制。方法:山药试管苗在试管块茎诱导培养基MS+6-BA 1.5 mg.L-1+NAA 1.5 mg.L-1+蔗糖5%中培养,取不同生长期的小块茎,采用ELISA法对其内源激素进行分离、纯化、测定。结果:在山药试管块茎形成过程中,GA3含量呈现倒"S"形变化;IAA含量总体呈下降趋势;ABA,ZR初期含量升高,后期下降;JA含量则一直上升;GA3与ABA和JA比值变化较为显著。结论:在山药试管块茎形成过程中,内源激素ABA,JA,IAA,ZR和GA3起着重要的调控作用。

Objective： Through analysis of variation and function of 5 main endogenous hormones in the formation of microtuber of Dioscorea opposite in vitro to explore the physiological and biochemical mechanism of microtuber development.Method： When microtubers were induced on MS＋6-BA 1.5 mg·L-1 ＋NAA 1.5 mg·L-1 ＋sucrose 5% medium,the endogenous hormones were isolated during different formation stages of microtubers,then purified and detected with enzyme-linked immunosorbent assays（ELISA）.Result： The results showed that GA3 slightly decreased in initial period,rose suddenly 20 days later,and than decreased.IAA showed a dropping tendency in the total course,ABA and ZR increased in a long period,dropped at last.JA continuously rose and never dropped,GA3 and ABA and the ratio of GA3 and JA varied obviously.Conclusion： IAA,ABA,JA,ZR and GA3 play an important role in controlling formation of microtubers in D.opposite in vitro.