附片指纹图谱研究及6种酯型生物碱含量测定

Study on HPLC fingerprinting and determine six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice

目的:采用高效液相色谱法建立白附片、黑附片的HPLC指纹图谱,并同时测定6种酯型生物碱的含量,为附子炮制和附片安全使用提供科学依据。方法:采用Phenomenex Gemini C18柱(4.6 mm×250 mm,5μm),乙腈-0.04 mol.L-1醋酸铵溶液(浓氨溶液调pH10.0)梯度洗脱。用国家药典委员会"相似度评价软件2004AB"处理分析,建立白附片、黑附片的HPLC指纹图谱,并测定白附片、黑附片、黄附片、生附子和盐附子中6种酯型生物碱的含量。结果:各批次白附片、黑附片指纹图谱相似度高,6种酯型生物碱分离度良好,黄附片、生附子指纹图谱与白附片、黑附片的对照图谱显著不同。结论:本研究所建立的方法可靠、简便易行。附片指纹图谱专属性强,结合6种酯型生物碱含量能较全面地反映其内在质量,为附片的安全使用及其质量控制提供科学依据。

Objective： To establish the HPLC fingerprint and determine six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice.Method： HPLC analysis was performed on a Phenomenex Gemini C18（4.6 mm×250 mm,5 μm）with 0.04 mol·L^-1 ammonium acetate（adjusted to pH 10.0 with ammonia water）and acetonitrile as mobile phase.Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine（2004 AB）was used in data analysis.Result： Baifupian,Heifupian from different samples were of high similarity in fingerprint,and the separation of six kinds of ester-type alkaloids was good.Huangfupian and crude aconite root showed significant difference in fingerprint,comparing with Baifupian and Heifupian.Conclusion： This method is simple and reliable.The HPLC fingerprint and contents of six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice can be used for their quality contro1.