藏药甲地然果(花锚)中酮成分的含量测定

Determination of xanthones in Tibetan herb Jiadiranguo (Herba Haleniae)

目的：建立HPLC同时测定椭圆叶花锚中4种[口山]酮成分[1-羟基-2,3,4,7-四甲氧基[口山]酮（1）,1-羟基-2,3,7-三甲氧基[口山]酮（2）,1-羟基-2,3,4,5-四甲氧基[口山]酮（3）,1-羟基-2,3,5-三甲氧基[口山]酮（4）]的含量的方法,并制定其含量限量标准。方法：采用Welchrom C18色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈-水（43∶57）,检测波长265 nm;流速1.2 mL.min-1,柱温40℃。结果：酮类化合物14分别在0.41416.580,1.7369.564,5.892117.84,3.012120.48 mg.L-1线性关系良好（r均为0.999 9）;4个对照品平均回收率（n=6）分别为102.5%,100.5%,97.9%,101.2%;采用该方法测定了30份自采或制药企业使用的椭圆叶花锚样品中上述4种成分的含量。结论：方法简便、准确,重现性好,可作为藏药椭圆叶花锚药材质量控制的方法;综合分析确定椭圆叶花锚中4种成分的含量之和不得低于1.80%。

A HPLC method was developed for simultaneous determination of 1-hydroxy-2,3,4,7-trimethoxyxanthone（1）,1-hydroxy-2,3,7-trimethoxyxanthone（2）,1-hydroxy-2,3,4,5-trimethoxyxanthone（3）,and 1-hydroxy-2,3,5-trimethoxyxanthone（4） in Halenia elliptica.The analytical column was Welchrom C18 column（4.6 mm×250 mm,5 μm）.The mobile phase was acetonitrile-water（43∶57）.The detection wavelength was 265 nm.The flow rate was 1 mL·min-1 and the column temperature was set at 40 ℃.There was good linearity between the peak areas and concentration at the ranges of 0.414-16.6,1.73-69.6,5.89-117,3.01-120.5 mg·L-1 for 1,2,3 and 4 respectively.The average recoveries（n=6） of 1,2,3 and 4 were 102.5%,100.5%,97.9% and 101.2%.Those four xanthones in thirty samples of H.elliptica.were determined by this method.The method is simple,accurate,repeatable,which could be used for the quality evaluation of H.elliptica..The total content of those four xanthones in H.elliptica should not less than 1.80% by comprehensive analysis.