胃肠安丸对肠易激综合征大鼠消化酶、水通道蛋白的影响

Effect of Weichang′an pill on intestinal digestive ferment and the AQP4 concentration in proximal colon in IBS-D rats

目的:探讨胃肠安丸对腹泻型肠易激综合征(IBS-D)大鼠止泻作用机制。方法:以喂饲高乳糖饲料加束缚应激相结合的方法复制IBS-D大鼠模型。将雌性Wistar大鼠,随机分为空白对照组、模型组及胃肠安(60 mg.kg-1.d-1)用药3组,每组各8只。通过比较体重增长率、腹泻潜伏期及腹泻指数,确定最佳用药周期。将45只大鼠随机等分为空白对照组、模型组、胃肠安80,60,40 mg.kg-1.d-1不同剂量等5组。计测胸腺指数、脾脏指数;小肠黏膜LDH,MDH及二糖(乳糖)酶活性;血清D-木糖含量;近端结肠组织AQP4含量。结果:用药后4 d胃肠安组与模型组体重增长率差值最大,腹泻指数差别有统计学意义(P<0.05),确定4 d为最佳用药周期。模型组较对照组胸腺指数、脾脏指数缩小(P<0.05),小肠黏膜LDH,MDH,二糖酶活性下降(P<0.05),AQP4含量升高(P<0.05)。与之相对,中剂量胃肠安组胸腺指数、脾脏指数增大(P<0.05),小肠黏膜LDH,MDH,二糖酶活性升高(P<0.05),但AQP4含量无显著差异。结论:胃肠安丸具有止泻作用,通过增强小肠黏膜消化酶活性,调节腹泻状态下机体糖分解代谢,是止泻机制之一。

Objective：To investigate the influence of Weichang′an pill on the treatment of diarrhea-predominant irritable bowel syndrome（IBS-D） in model rats.Method： Animal model of compound diarrhea was induced by a lactose enriched diet in the Wistar rat,combining with restraint stress.Twenty four female Wistar rats were randomly divided into normal group,model group and 60 mg·kg^-1·d^-1 Weichang′an pill group.The rate of weight increase,the incubation period of diarrhea and the diarrhea index were observed.Then 45 female Wistar rats randomly divided into five groups： control group,model group and Weichang′an pill groups of high,medium and low doses（80,60,40 mg·kg^-1·d^-1）.The indexes of thymus and spleen were calculated.The activities of LDH,MDH and disaccharidase in intestinal organization were inspected.Serum D-xylose content and the AQP4 concentration in proximal colon were detected.Result： After taking Weichang′an pill for 4 days,the rate of weight increase in Weichang′an pill group was higher than the model group′s.While the rate of diarrhea was lower significantly.So the best cycle of taking medicine was 4 days.The indexes of thymus and spleen of model group were decreased than that of control group.And the activities of LDH,MDH and disaccharidase in intestinal organization were also decreased.But the AQP4 concentration in proximal colon was increased.Compared with the model group,the indexes of thymus and spleen increased remarkably in the group of medium doses.Meanwhile,the activities of LDH,MDH and disaccharidase increased.But the AQP4 concentration didn′t change.Conclusion： Weichang′an pill has the effect of antidiarrhea.It can adjust the sugar＇s catabolism through increasing the activity of intestinal digestive ferment.