丹参酮对维甲酸致小鼠骨质疏松的防治作用

Effect of tanshitone on prevention and treatment of retinoic acid induced osteoporosis in mice

目的:研究丹参总酮对维甲酸致小鼠骨质疏松的防治作用,并探讨其主要作用机制。方法:维甲酸(90 mg.kg-1)致小鼠骨质疏松模型,丹参酮(40,80,160 mg.kg-1)灌胃给药,实验过程监测体重,2周后以比色法测定血清钙、磷含量和碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)活性,竞争放射免疫法测定血清中骨钙素(BGP)和雌二醇(E2)含量,取股骨进行骨组织形态学观察。结果:维甲酸可引起高转换型骨质疏松,丹参酮可升高E2值,降低P,ALP,TRAP,BGP水平。结论:丹参酮对维甲酸致小鼠骨质疏松有一定的防治作用,其机制可能与其减缓雌激素水平降低,抑制骨高转换有关。

Objective： To observe the prevention and therapeutic effects of tanshitone（TAN） on retinoic acid induced osteoporosis in mice.Method： The mice osteoporosis was induced by given retinoic acid intragastrically for two weeks.The histomorphological features of bone were observed and biochemical indexes in serum（Ca,P,ALP,TRAP,E2,BGP） were determined after the mice were given TAN at the dose of 40,80,160 mg·kg^-1 respectly.Result： Tanshinone can induce high conversion of osteoporosis.The levels of P,ALP,TRAP and BGP in the TAN groups were lower than the model group,while the E2 level was higher than the model group.Conclusion： Tanshitone can prevent the loss bone in the experimental mice.The machanism may be that it improves the level of estrogenic hormone and inhibits the high bone turnover.