黄芪对兔纤维环细胞增殖及凋亡的调节作用

The regulatory effects of Astragalus membranaceus on apoptosis and proliferation of rabbit annulus fibrosus cells in vitro

目的:探讨黄芪对压力损伤的兔纤维环细胞增殖及凋亡的影响。方法:藻酸盐凝胶培养兔纤维环细胞,以1Mpa持续气压静压力诱导纤维环细胞损伤,在培养基内分别加入10,50,100,500,1000mg.L-1的黄芪注射液,培养48h后CCK-8法检测细胞增殖情况,AnnexinV-PI染色检测细胞凋亡情况。结果:(1)CCK-8显示,各浓度的黄芪注射液能促进纤维环细胞的增殖,其中50mg.L-1黄芪注射液的作用最显著(与空白对照相比,P<0.01);(2)AnnexinV-PI染色显示,50mg.L-1黄芪注射液降低纤维环细胞凋亡率的作用最为显著(与空白对照相比,P<0.01),但当浓度增加至1000mg.L-1时,凋亡率虽较空白对照有所降低,但差异无显著性(P>0.05)。结论:黄芪注射液对损伤的纤维环细胞有促进增殖和抑制凋亡作用,具备用于纤维环细胞保护的潜力。

OBJECTIVE To investigate the regulatory effect of Astragalus memdranaceus on apoptosis and proliferation of rabbit annulus fibrosus cells which impaired by static gas pressure. METHODS Rabbit annulus fibrosus cells were cultured in alginate gel and impaired by 1 Mpa static gas pressure, at the same time, the Astragalus mernbranaceus injection of the does 10, 50,100,500, 1 000 mg·L^-1 was added to each group. After 48 hours, the proliferation of cells was evaluated by CCK-8 method, and the cells were stained with Annexin V- PI to evaluate apoptosis using flow cytometry. RESULTS ①CCK-8 displays： Compared with the control group, the A.stragalus membranaceus injection can promote the proliferation of annulus fibrosus cells, to improve the vitality of cells, and the significiant effect does is 50 mg· L^-1 （P〈0. 01. ②Annexin V- PI staining reveals： Compared with control group, the does of 50 mg·L^-1 Astragalus rnernbranaceus injection can significantly reduce the apoptosis of annulus fibrosus cells（P〈0.01）. However, when the concentration increased to 1 000 mg·L^-1 , the apoptosis rate decreased, but no statistical significance（P〉0. 05）. CONCLUSION Astragalus membranaceus injection can protect impaired annulus fibrosus cells, promoting proliferation and reducing apoptosis in vitro, which has the potential for annulus fibrosus protection.