2009～2010年乌鲁木齐市流行性感冒病原学监测分析

Analysis of Pathogenic Surveillance of Influenza in Urumqi City from 2009 to 2010

目的了解流行性感冒病毒（流感）的流行情况,探讨流感流行规律,为制订流感控制措施提供依据。方法对乌鲁木齐市监测门诊及疫情点的疑似流感样病例采集咽拭子标本,用核酸检测和接种狗肾传代细胞（MDCK）培养进行流感病毒分离及进行血凝试验,并对阳性者进行血凝抑制试验进行分型鉴定。结果 2009年10月-2010年3月监测流感样标本与咽拭子标本共1 218份,分离出流感病毒181株,分离率3.72%,分别为甲型H1N1亚型8株、H3N2亚型32株、新甲型H1N1 104株、B型37株。结论 2009年10月-2010年1月是以新甲型H1N1亚型流感病毒流行为主,2010年2月以B型流感流行为主。

Objective To understand epidemic situation of influenza virus and discuss regular pattern of influenza prevalence,and provide basis for prevention and control of influenza.Methods Swabs specimens of suspected influenza-like cases from monitoring clinic and epidemic point in Urumqi were collected with DNA testing and cultivation by vaccinating renal cell MDCK of dogs for influenza viruses separation and blood clots test.The positives were differentiated with hemagglutination inhibition assay.Results There were 1 218 specimens of influenza pandemic sample and swabs specimens monitored from October 2009 to March 2010,and 181 influenza virus strains were isolated（3.72%）,including new influenza H1N1 subtype of 8 strains,and H3N2 subtype of 32 strains,new H1N1 of 104 strains and hepatitis B of 37 strains.Conclusions The predominant virus type in influenza seasons is new influenza pandemic influenza H1N1 subtypes from October 2009 to January 2010 and influenza B in February 2010.