贵州彝族 瑶族及汉族人群乙型肝炎病毒感染与趋化因子受体5基因多态性的相关性研究

Genetic association of CCR5 59029 polymorphisms with HBV infection in Han nationality,Yi and Yao minorities of Guizhou

目的研究CCR5 59029等位基因在贵州世居少数民族(彝、瑶族)以及汉族人群的分布,并分析CCR5位点突变与乙型肝炎病毒(HBV)的关系。方法 2009年2月至5月,采用PCR-RFLP技术分析贵州92份黔西彝族、101份威宁彝族、138份荔波瑶族以及165份毕节汉族CCR5启动子区59029位点多态性,抽取样本进行DNA测序验证。结果贵州省黔西彝族、威宁彝族、荔波瑶族及毕节汉族的CCR559029位点基因型频率分别为:纯合突变型(AA)为20.65%、17.82%、8.7%、22.42%;杂合子(AG)为43.48%、44.55%、34.78%、44.85%;野生型(GG)为35.87%、37.62%、56.52%、32.37%。等位基因频率:A为26.09%～44.85%,G为55.15%～73.91%。基因型频率在荔波瑶族和民族总体人群感染组与非感染组之间分布有统计学意义(P<0.05),等位基因频率在威宁彝族、荔波瑶族人群感染组与非感染组之间分布差异有统计学意义(P<0.05)。结论 CCR5 59029位点可能对贵州省汉、瑶、彝族人群HBV感染具有保护性,CCR5基因可能是HBV感染的拮抗基因之一。

Objective To investigate the distribution of CCR5 59029 polymorphisms in Han nationality, Yi and Yao minorities of Guizhou and the relationship between the CCR5 mutation and HBV infection. Methods The CCR6 promotor 59029 polymorphisms were analyzed with PCR-RFLP in 92 subjects of Yi nationality in Qianxi, 101 of Yi nationality in Weining, 138 of Yao nationality in Libo and I65 of Han nationality in Bijie from Guizhou province. The findings were tested with DNA sequencing. Results The genotype frequecies of CCR5 59029 in Qianxi Yi Minority, Weining Yi Minority, Libo Yao Minority and Bijie Hans were 20. 65% , 17. 82% , 8.7% and 22.42% for homozygous mutated variants （AA） ;43.48% ,44. 55% ,34. 78% and 44. 85% for heterozygous mutated variants （AG） ;35.87% ,37.62% ,56. 52% and 32. 37% for wild-type variants （GG）. Allele frequencies for A ranged from 26. 09% to 44. 85% and from 55.15% to 73.91% for G. The genotype frequency differed signficantly in the general population, Libo Yao Minority between non-infected and infected subjects （ P 〈 0. 05）. The allele frequencies also differed significantly in Weining Yi Minority and Libo Yao Minority between non-infected and infected subjects （ P 〈 0. 05 ）. Conclusion CCR5 59029 may be protective against HBV among population of Han nationality,Yao and Yi minorities in Guizhou Province. CCR5 may be one of antagonist genes for HBV infection.