卵巢上皮性癌组织中PAX2基因的表达及临床意义

Expression and Clinical Significance of PAX2 in Epithelial Ovarian Cancer

目的探讨PAX2mRNA和蛋白在卵巢上皮性癌中的表达及临床意义。方法采用逆转录聚合酶链反应和免疫组化方法检测PAX2mRNA和蛋白在31例卵巢上皮性癌、11例卵巢上皮性交界性肿瘤、10例卵巢上皮性良性肿瘤和11例正常卵巢组织中的表达,并分析其表达与卵巢上皮性癌临床病理学指标的关系。结果正常卵巢表面上皮无PAX2的表达,PAX2mRNA和蛋白在卵巢上皮性癌组织中的表达显著高于卵巢交界性肿瘤组织、卵巢良性肿瘤组织及正常卵巢组织,差异均有统计学意义(P<0.01);卵巢交界性肿瘤组织、卵巢良性肿瘤组织及正常卵巢组织中PAX2mRNA和蛋白表达的差异无统计学意义(P>0.05)。在晚期(Ⅲ～Ⅳ)及低分化的卵巢上皮性癌中PAX2mRNA和蛋白的表达高于早期(Ⅰ～Ⅱ)及高中分化者,差异均有统计学意义(P<0.05)。结论 PAX2在卵巢癌的发生发展中起一定作用,可能对卵巢癌恶性程度的评估有指导意义。

Objective To investigate the expression and clinical significance of PAX2 in epithelial ovarian cancer.Methods The expression levels of PAX2 mRNA and protein in 31 ovarian epithelial carcinomas,11 borderline ovarian tumors,10 benign ovarian tumors,and 11 normal ovarian tissues were examined by reverse transcription-polymerase chain reaction （RT-PCR）and immunohistochemistry.Results There were no PAX2 mRNA and protein expression in normal ovarian tissues.The expression of PAX2 mRNA and protein in epithelial ovarian cancer were significantly higher than those in borderline ovarian tumors,epithelial benign tumors and normal ovarian tissues respectively（P 〈 0.01）.There was no significant difference among normal ovarian tissues,epithelial benign tumors and borderline ovarian tumors （P 〉 0.05）.Furthermore,there were significant differences between high grade（G1,G2）and low grade（G3）（P 〈 0.01）,advanced stage（Ⅲ,Ⅳ）and early stage（Ⅰ,Ⅱ）（P 〈 0.05）.Conclusion PAX2 played an important role in the genesis and development of ovarian tumors.PAX2 might be related to tumorigenesis and development of epithelial ovarian caicinoma.