恒温扩增技术在动物疫病病原检测中应用现状及展望

Current Status and Prospects of Isothermal Amplification in the Detection Pathogens of Animal Disease

介绍了链置换扩增技术、转录介导扩增技术、核酸序列依赖性扩增技术、环介导等温扩增技术、赖解旋酶恒温基因扩增技术、滚环扩增等六种恒温扩增核酸的技术的特点及其研究应用概况,并对恒温扩增技术的前景予以展望,以期为这些技术的应用提供参考。

In the paper,an overview of the current status relating to isothermal amplifications including strand displacement amplification,transcription mediated amplification,nucleic acid sequence-based amplification,loop-mediated isothermal amplification,helicase-dependant amplification and rolling circle amplification were reviewed,and the features,applications,the challenges and the techniques problems of them were discussed.The prospects and further applications of isothermal amplifications in their future development were considered,too.