运动预处理对力竭运动大鼠大脑皮质GFAP表达的影响

Effect of Exercise Preconditioning on Exhaustive Exercise Induced the Expression of Glial Fibrillary Acidic Protein in Rats Cerebral Cortex

目的:建立SD大鼠运动预处理模型和一次性力竭运动模型,探讨运动预处理对一次性力竭运动诱导的大鼠大脑皮质神经胶质酸性蛋白表达的影响。方法:将30只SD雄性大鼠随机分为3组:对照组(C组),力竭运动组(EE组),运动预处理组(EP组),EP组采用持续4周的中等游泳负荷训练后,EE组和EP组负自身体重的3%进行一次性力竭运动游泳,用免疫组织化学法及免疫印迹法检测大鼠大脑GFAP的表达。结果:EE组GFAP表达强度较C组无显著性差异(P>0.05),EP组较EE组及C组显著增强(P<0.05);EP组大鼠力竭游泳时间较EE组明显延长(P<0.05)。结论:一次性力竭运动大脑中GFAP未见明显活化,可能参与了中枢性运动疲劳的病理生理过程,而运动预处理后GFAP可显著活化,这可能是其提高运动能力,缓解中枢性运动疲劳的机制之一。

Objective：Established SD rat＇s exercise preconditioning models and exhaustive exercise models to explore the influence of exercise preconditioning on exhaustive exercise induced the glial fibrillary acidic protein expression in the cerebral cortex of rats.Method：30 male SD rats were divided into three groups randomly,control group（group C）,exhaustive exercise group（group EE） and exercise preconditioning group（group EP）.First,group EP carries out a moderate exercise preconditioning for 4 weeks,then group EE and group EP carry out an exhaustive swimming loading with 3% body weight.Immunohistochemical and western blotting ways were taken to detect the glial fibrillary acidic protein expression in the cerebral cortex of rats.Results：There were not significant difference of GFAP expression,the EE group and the C group（P0.05）.However,there were significant difference when EP group vs both C group and EE group（P0.05）,there were significant difference of an exhaustive swimming time between EP group and EE group（P0.05）.Conclusion：There is no significant change of GFAP expression,when rats suffered with an exhaustive exercise which may due to the central fatigue.However,exercise preconditioning can significant activation the GFAP,which maybe one of the mechanism of enhancing the exercise ability and relieving the central fatigue.