猴头菌液体发酵培养基优化提高菌丝生物量的研究

Mycelium of Liquid Fermentation Medium Optimization to Improve the Biomass of Hericium Erinaceus

目的:研究猴头菌液体培养提高菌丝生物量的最佳配方。方法:通过对猴头菌液体发酵的各常用碳源与氮源单因素试验和正交试验L_9(3(?)4),研究了葡萄糖、玉米粉、淀粉、蔗糖、酵母粉、蚕蛹粉、蛋白胨、黄豆粉对提高菌丝生物量的影响。结果:葡萄糖、玉米粉复合碳源为最适碳源,蛋白胨、黄豆粉为最适氮源,从而得到了猴头菌液体发酵培养的最佳培养基为:葡萄糖30g/L、玉米粉30g/L、蛋白胨4g/L、黄豆粉15g/L、KH_2PO_41.5g/L、MgSO_40.75g/L;培养条件为:温度25℃,时间为(140～144)h,在最佳培养工艺条件下,猴头菌丝体的收率为28.1g/L,比基础工艺的菌丝收率提高了一倍多,菌丝体中的多糖、多肽含量分别为基础工艺的1.34倍与1.12倍。结论:通过正交试验得到的培养基配方菌丝生物量收率高,有效成分多糖、多肽含量高,工艺稳定,可作为猴头菌液体发酵的生产工艺。

Objective：To study the liquid culture of Hericium erinaceus mycelium biomass to improve the best formula.Methods： Fermentation Hcricium commonly used carbon and nitrogen in various single-factur experiments and orthogonal test L9 （3^4）,of glucose,maize flour, starch,sugar,yeast powder, silkworm chrysalis powder,peptone,soybean flour to improve the mycelium biomass.Results：glucose,corn flour as carbon sources for the optimal carbon source,peptone,soybean powder The Optimal nitrogen source,which has been submerged cultivation of Hericium the best medium： glucose 30g/L,com flour 30g/L,peptone 4g/L,soy flour 15g/L,KH2PO4 l.5g/L,MgSO4 0.75g/L;cultural conditions were： temperature 25℃,time for the 140 -144 h,the optimum culture conditionsithe Hericium mycelium yield was 28.1g/L,based on technology than the yield of mycelia has doubled and more mycelium polysaccharide,polypeptide content-based technique was 1,34 times and 1.12 times： Conclusion：get the medium orthogonal formula mycelium biomass yields and effective component of polysaccharidelpolypeptide content was high,process stability, as Hericium liquid femlentation production process.