慢性复合应激对大鼠胰岛β细胞功能的影响

Effects of Chronic Multiple Stress on Pancreas β Cell Function in Rats

[目的]研究慢性复合应激对大鼠胰岛β细胞的影响。[方法]将40只SD大鼠随机分成应激组和对照组,每组20只。应激组实施8周的慢性复合应激刺激,对照组在此期间正常饲养。检测两组大鼠血糖、胰岛素,口服糖耐量实验(OGTT),计算胰岛素分泌指数(HOMA-β)、OGTT曲线下面积、早期相胰岛素分泌指数(ΔI30/ΔG30)、β细胞功能修正指数(MBCI),并检测胰岛β细胞凋亡情况。[结果]慢性复合应激8周后,与对照组相比,应激组大鼠空腹血糖明显升高(P<0.001),空腹胰岛素水平和HOMA-β明显降低(分别为P<0.05,P<0.01);同时与对照组相比,应激组大鼠葡萄糖曲线下面积明显增加(P<0.05)、胰岛素曲线下面积、ΔI30/ΔG30以及MBCI明显减少(分别为P<0.05,P<0.05,P<0.01)。细胞凋亡结果显示应激组大鼠凋亡率明显高于对照组(P<0.001)。[结论]慢性复合应激使大鼠胰岛β细胞功能降低。

[Objective] To explore the effect of chronic multiple stress on pancreas βcell function in rats.[Methods] Forty male Sprague-Dawley rats were randomly divided into stress and control groups with twenty in each.Plasma glucose,insulin, insulin resistance,βcell function,and oral glucose tolerance tes（tOGTT）were measured and calculated.βcell apoptosis were detected using Tunel measurement.[Results] Compared with the control group,the stress group had significantly higher fasting plasma glucose（FPG）and increased area under the curves of blood glucose levels（AUCG）（P0.001,P0.05）.The fasting insulin（FINS）,homeostasis model assessment（HOMA）-β,the areas under the curves of serum insulin levels（AUCI）,ΔI30/ ΔG30 and modifiedβcell index（MBCI）were significantly lowe（rP0.05,P0.01,P0.05,P0.05,P0.01）in the stress group than the control group.The apoptosis rate of the stress group was significantly higher than the control group（P0.001）. [Conclusion] It is observed that chronic multiple stress reduced the pancreasβcell function in rats.