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水体中微囊藻毒素和节球藻毒素的UPLC-MS^n分析方法研究 被引量：2

Analysis of Microcystins and Nodularin in Water by Ultra Performance Liquid Chromatography/Tandem Mass Spectrometry
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摘要 [目的]针对水体中3种代表性的微囊藻毒素(microcystin-RR,MC-RR;microcystin-YR,MC-YR;microcystin-LR,MC-LR)和节球藻毒素(nodularin,NOD),建立一种快速、准确、可靠的痕量分析方法。[方法]样品经C18固相萃取柱净化富集后,采用超高效液相色谱-电喷雾串联三重四级杆质谱法(ultra performance liquid chromatography/tande mmass spectrometry,UPLC-MS/MS),在多反应监测(Multi reaction monitoring,MRM)模式下进行检测,采用基质匹配标准曲线法消除基质效应。[结果]该方法加标回收率为95.7%~115.0%,精密度(RSD)范围为2.43%~6.04%,检测限可达0.05~0.20ng/L。将该方法应用于实际水样分析,水体中3种微囊藻毒素和节球藻毒素含量低于检测限。[结论]建立了水体中3种痕量微囊藻毒素和节球藻毒素的UPLC-MS/MS分析方法,该方法可用于实际水样的检测。 [Objective] To establish an analytical method for the determination of trace content of microcystins（MC-RR, MC-YR and MC-LR）and nodularin（NOD）in water sample.[Methods] After cleaned and enriched by solid phase extraction （SPE）using C18,the water sample was determined by ultra performance liquid chromatography/tandem mass spectrometry（UPLC- MS/MS）in multi-reaction-monitoring mode.Matrix-matched standard curves were used to compensate the matrix effect.[Results] The recoveries were in range of 95.7%-115.0%with relative standard deviation（RSD）2.43%-6.04%.The detection limits were 0.05-0.20ng/L.[Conclusion] An analytical method for the determination trace microcystins and nodularin in water sample was established by UPLC-MS/MS.This method could be used to analyze local water samples.
作者 何海波 时肖宁 刘敏
机构地区 上海大学理学院 上海市检测中心生物与安全实验室
出处 《环境与职业医学》 CAS 北大核心 2010年第10期626-629,共4页 Journal of Environmental and Occupational Medicine
基金 上海市自然科学基金资助项目(编号:08ZR1416600)
关键词 超高效液相色谱-电喷雾三重四级杆串联质谱 多反应监测 微囊藻毒素 节球藻毒素 基质匹配标准曲线法 水 UPLC-MS/MS multi-reaction-monitoring microcystins nodularin matrix-matched standard curves water
分类号 R115 [医药卫生—公共卫生与预防医学]
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参考文献12

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共引文献92

同被引文献64

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环境与职业医学
2010年 第10期

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