CO_2气腹对裸鼠腹腔种植肿瘤MMP-2和TIMP-1蛋白表达的影响

Effects of CO_2 Pneumoperitoneum on Protein Expression of MMP2 and TIMP-1 of peritoneal implanted tumor in a nude mouse model

目的评价CO2气腹及不同气腹压力对裸鼠腹腔种植肿瘤基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶抑制剂-1(TIMP-1)蛋白表达的影响。方法 30只裸鼠建立腹腔种植肿瘤模型,造模成功后随机分组:对照组、5mmHg和10mmHgCO2气腹组,气腹作用时间1h。气腹干预后1周检测肿瘤组织MMP2、TIMP-1蛋白表达情况。结果与对照组比较,气腹干预后1周5mmHg和10mmHgCO2气腹组肿瘤组织MMP-2蛋白表达均显著升高(P<0.01),且10mmHg组高于5mmHg组(P<0.01)。两组TIMP-1表达降低,且10mmHg组低于5mmHg组(P<0.01)。MMP-2/TIMP-1比例下降,10mmHg组下降更为显著(P<0.01)。结论 :CO2气腹作用后早期可使肿瘤组织MMP-2升高,TIMP-1下降,气腹压力高作用更为明显。

Objective To evaluate the effects of CO2 pneumoperitoneum and different pneumoperitoneum pressure on protein expression of MMP2 and TIMP-1 of peritoneal implanted tumor.Methods Thity nude mouse were divided randomly into three groups after the implanted tumor moel was established（control group,5 mm Hg and 10 mm Hg CO2 pneumoperitoneum,1 h）.The correlated cytokines of aggression（MMP2/TIMP-1）in tumor tissue were detemined one week after pneumoperitoneum intervention.And to evaluate the effects of different CO2 pneumoperitoneum pressure on protein expression of MMP-2 and TIMP-1.Results Compared with the control group,the protein expression level of MMP-2 in the two CO2 pneumoperitoneum groups increased significantly in early post-operation（one week）（P0.01）.Moreover the expression level of MMP-2 in 10 mm Hg group was higher than that in 5 mm Hg group pneumoperitoneum（P0.01）.The protein expression of TIMP-1 decreased in both groups,with the lower expression in 10 mm Hg group（P0.01）.And the The expression level of MMP-2/TIMP-1 decreased after CO2 pneumoperitoneum intervention,especially in 10 mm Hg group（P0.01）.Conclusion The expression of MMP-2 increase and TIMP-1 decrease in the early period after CO2 pneumoperitoneum intervention.The higher pressure has more significant effects.