用旋转式荧光定量PCR分析甲型H1N1流感病毒传播的特点

Application of the Rotary Fluorescent Quantitative PCR in Epidemiology Characteristic of Influenza A/H1N1

目的:探讨旋转式荧光定量PCR仪在检测临床标本中的应用价值。方法:通过旋转荧光定量-PCR技术对可疑病例的咽拭子、鼻拭子进行检测。结果2009年检测出甲流患者791例,检出率为38.79%(791/2039);甲型H1N1 157例,检出率为7.7%(157/2039),2010年1-6月检测出甲型H1N1为21例,检出率为1.44%(21/1455)。结论本地区甲型H1N1流感是以普通甲流感染为主,甲型H1N1流感混在其中,本地区的传播高峰是在2009年10月,甲型H1N1流感病毒在2010年来传播呈明显减弱趋势。

Objective： The application of rotary fluorescent quantitative PCR instrument in clinical detection. Method： Pharynx and nose swabs of 3494 doubtful cases were detcted with Line-geneK-FQ-PCR. Results： Influenza A patients were 791, and detection rate was 38.79%（791/2039）. Patients who in fected H 1N 1 virus were 157, and detection rate was 7.7%（157/2039）. Patients who infected H1N1 virus were 21 in 2010.l-2010.6, and detection rate was 1.44%（21/1455）. Conclusion： Spreading peak of influenza A/H 1N 1 occurred in October 2009. Transmiting tendency obviously weakened from 2010.