补骨脂素对淀粉样蛋白损伤体外神经元的保护作用研究

Protection of Psoralen on β Amyloid-induced Neuron in Vitro

目的:探讨补骨脂素对淀粉样蛋白损伤体外神经元的雌激素样保护作用及其机制。方法:β淀粉样蛋白(Aβ)处理体外大鼠海马神经元建立体外Aβ损伤模型,考察补骨脂素对Aβ对神经元MTT、LDH漏出及选择性老年痴呆症指示因子1(sela-din-1)mRNA表达的影响。结果:补骨脂素可以拮抗Aβ对神经元细胞的损伤,上调seladin-1的表达。结论:上调seladin-1的表达可能是补骨脂素拮抗Aβ神经毒性的机制之一。

Objective： This study examined an estrogen- like protection of psoralen on β amyloid -induced neuron in vitro. Methods： We examined that the effect of psoralen on cell viability, the production of Lactate dehydrogenase （LDH） and seladin - 1 mRNA expression induced by β amyloid （Aβ ） treatment in rat primary cultured hippocampus neuron. Results： Psoralen could significantly increased cell viability and seladin - 1 mRNA expression induced by A~ treatment in rat primary cultured neuron. Conclusion： Psoralen could protected neuron against Aβ - induced injury. Increasment of seladin - 1 mRNA expression might be a mechanism underlying the neuroprotective activity of it.