摘要
目的:观察补肾活血合剂对糖尿病(DM)阳痿(ED)大鼠阴茎平滑肌组织中TGF-β1,a-SMA的影响。方法:将实验小鼠用2%链脲佐菌素液(STZ)按60mg/kg,腹腔注射建立糖尿病模型,然后在每只糖尿病模型大鼠颈项处注射阿朴吗啡(AP0sigma公司)80μg/kg,录相记录阴茎勃起次数。筛选DM性ED模型,将造成DM性ED模型随机分为正常组,模型组,中药高、低剂量组,达美康组,达美康+安雄组。除正常组外,其余各组连续给药84d,然后处死测定阴茎平滑肌组织中TGF-β1,a-SMA的改变。结果:补肾活血活剂对TGF-β1,a-SMA有改善作用。结论:补肾活血合剂对糖尿病阳痿大鼠阴茎平滑肌组织中TGF-β1,a-SMA有明显保护效果。
Objective： Observing the effects of reinforcing kidney and activating blood recipe affecting the value of TGF -β1 and α- SMA in the tissue of corpus cavernosum Smooth muscle of DMED - rats. Methods ： An animal model of diabetes was induced by a single intravenous dose of streptozotocin （STZ, 60 mg/kg body weight） in Wistar rats. Recording the numbers of penile erection of each rat by camera after subcutaneous inject of apomorphine （80 mg/kg）. Screening the model of STZ - rats with erectile dysfunction and grouping into 6 groups ： control group, model group, Chinese Medicine group, low - dosage group, Diamicron group, Diamicron combined Testosterone Undecanoate group. After administrating 12 weeks, observed the change of TGF- β1 and α- SMA in the tissue of corpus cavernosum smooth muscle of DMED - rats. Results ： The reinforcing kidney and activating blood recipe can improve the vulue of TGF- β1 and α- SMA. Conclusion： The reinforcing kidney and activating blood recipe has significant protective effect of TGF- β1 and α - SMA in the tissue of corpus cavernosum smooth muscle of DMED - rats.
出处
《中医学报》
CAS
2010年第6期1113-1116,共4页
Acta Chinese Medicine
基金
贵州省中医药管理局重点项目(黔中发2005-30)
关键词
TGF-β1
α-SMA有明显保护效果
reinforcing kidney and activating blood recipe
diabetes mellitus
erectile dysfunction
TGF - β1
α - SMA
immunhisto-chemistry
