腺苷A_1受体拮抗剂DPCPX对低氧复氧脑星形胶质细胞影响的实验研究

Effect of adenosine A_1 receptor antagonist DPCPX on cerebral astrocytes after hypoxia and reoxygenation

目的:观察腺苷A1受体拮抗剂1,3-二丙基-环戊黄嘌呤(8-cyclopentyl-1,3-dipropylxanthine,DPCPX)对体外培养低氧复氧(hypoxia and reoxygenation,H/R)大鼠脑星形胶质细胞促红细胞生成素(erythropoietin,EPO)释放量、谷氨酰胺合成酶(glutamine synthase,GS)、超氧化物歧化酶(superoxide dimutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)活性的影响,探讨腺苷A1受体在H/R星形胶质细胞神经保护中的作用。方法:对大鼠大脑皮层星形胶质细胞进行体外培养并建立H/R模型,在低氧同时加入终浓度为100nmol/L DPCPX12h后复氧1,6,12,24h,检测EPO释放量,GS,SOD和GSH-PX活性的变化。结果:与对照组比较,DPCPX组H/R星形胶质细胞的EPO释放量显著减少;GS,SOD和GSH-PX的活性降低。结论:腺苷A1受体参与H/R星形胶质细胞的神经保护作用。

Objective To study the effects of adenosine A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine（DPCPX） on the release of astrocytic erythropoietin （EPO）,the activities of glutamine synthase （GS）,superoxide dimutase （SOD） and glutathione peroxidase （GSH-PX） after hypoxia and reoxygenation （H/R） in rats,and to explore its role in the astrocytic neuroprotection.Methods Primary cultured rat cortical astrocytes and the H/R model was established.100 nmol/L DPCPX was added into media at the beginning of hypoxia and at 1,6,12,24 h after reoxygenation.The release of EPO,the activities of GS,SOD and GSH-PX were measured.Results Comparing with the H/R group,the release of astrocytic EPO,the activities of GS,SOD and GSH-PX was significantly decreased in the DPCPX group.Conclusion Inhibition of adenosine A1 receptor increases the H/R injury in astrocytes.