影响纤维蛋白原检测结果的因素分析

Analysis on factor influencing fibrinogen detection

目的:探讨纤维蛋白原测定的影响因素。方法:采用凝血酶法检测不同样本状态纤维蛋白原含量,并对纤维蛋白原测定的两种方法即凝血酶法和PT-演算法进行方法学比对研究。结果:标本溶血或脂血不影响利用凝血酶原理对纤维蛋白原含量的测定,但PT-演算法对脂血标本则产生测定误差;方法学比对结果显示纤维蛋白原含量在1.5-4.3g/L浓度之间,两种方法学之间无统计学差异,但对于过低或过高浓度时两种方法测定结果产生明显的偏离。结论:在临床工作中首先要采集合格的标本,然后尽可能选择凝血酶法进行测定,以确保为临床提供准确可靠的实验结果。

Objective： To investigate the influence factor of fibrinogen detection. Methods： Fibrinogen content in different sample states was detected with thrombin assay; two methods （thrombin assay and PT-computation） that determine the fibrinogen were studied by using methodology intercomparison. Result： Sample hemolysis or lipohemia did not affect the dedtermination to fibrinogen content with prothrombin, but there are determinate error to sample lipohemia with PT-forward algorithm; When the result of contrast test display that the fibrinogen content is 1.5-4.3g/L concentration,the two kinds contrast test is not no statistical difference,but the determination results with two constrast test produce significantly deviation in over lowering or high concentration. Conclusion： In order to provide accurate and reliable experiment results for clinical work,we should collect qualified samples and then choose thrombin assay as much as possible.