原发性干燥综合征患者外周血EB病毒标志物检测及意义

Detection and Significance of Epstein Barr Virus Markers in Peripheral Blood of Patients with Primary Sjogren's Syndrome

目的:探讨原发性干燥综合征(primary Sjfigren's syndrome,pSS)患者外周血EB病毒(Epstein Barr virus,EBV)标志物的检测及意义。方法:应用实时荧光定量PCR技术检测29例原发性干燥综合征患者和44例正常对照组外周血单个核细胞(peripheral blood mononuclear cell,PBMC)EBV DNA,酶联免疫吸附法(ELISA)检测患者和正常对照血清EBV衣壳抗原(viral capsid antigen,VCA)特异性IgM、IgG抗体以及EBV早期抗原(early antigen,EA)IgG抗体。结果:pSS患者组及正常对照组EBV DNA拷贝数分别为26.76±36.00copies/μgDNA和7.41±12.02copies/μgDNA,统计学分析表明pSS患者组EBV拷贝数明显高于正常对照组(t=3.603,P<0.01)。患者组血清VCA-IgM和EA-IgG阳性率分别为20.69%(6/29)和62.07%(18/29),正常对照组则均为2.27(1/44),两两比较均明显高于正常对照组(P<0.05,P<0.01)。患者组血清VCA-IgM水平与正常对照组比较无显著性差异,而EA-IgG抗体水平明显高于正常对组(P<0.01)。结论:原发性干燥综合征患者存在EBV的激活感染,EBV激活感染是导致患者免疫功能异常的重要原因,潜伏EBV的激活与干燥综合征的发病有关。

Objective： To study the significance of Epstein Barr virus markers in peripheral blood of patients with primary Sjogren＇s Syndrome（pSS）. Methods： We detected EBV DNA in the PBMC （peripheral blood mononuclear cell） of 29 pSS patients and 44 normal controls by Real-time PCR,as well as EBV VCA （viral capsid antigen）-specific IgM, IgG and EBV EA （early antigen） IgG by ELISA. Results： The EBV DNA copy number in pSS patients and normal controls were 26.76±36.00 copies/μg DNA and 7.41±12.02 copies/μg DNA, respectively. The statistics results indicated EBV DNA copy number in pSS patients was higher than that in normal conrols （t=3.603,P〈0.01）. The VCA-IgM and EA-IgG positive rate in pSS patients were 20.69% （6/29） and 62.07% （18/29）, while that in normal controls were 2.27%（1/44）, 2.27%（1/44）, there was obvious difference between two groups by statistics analysis（P〈0.05, P〈0.01）. Conclusions： There is EBV active infecton in pSS, which plays an important role in immune dysfunction in pSS patients. The activation of latent EBV is associated with the pathogenesis of pSS.