浙江十种经济海藻的氨基酸含量分析

Reversed-phase high performance liquid chromatographic PICO-TAG analysis of Amino Acids in 10 seaweeds of Zhejiang Province

采用以异硫氰酸苯脂（ＰＩＴＣ）为柱前衍生化的ＰＩＣＯ－ＴＡＧ反相高效液相色谱法对浙江沿岸１０种经济海藻的氨基酸含量进行测定，得出以上海藻的氨基酸色谱图。发现１０种海藻的氨基酸组成基本相同。氨基酸总含量达１０．５７％～３３．５１％，其中必需氨基酸占总量的２８．２８％～４５．２２％。该测定结果可以为海藻经济开发提供参考。

The analysis of amino acids in 10 seaweeds of Zhejiang Province were made with h igh performance liquid chromatography with PICO TAG method. The contents of sev enteen amino acids were determined. The sum of amino acids were between 33 51 m g ( Grateloupia ramosissima) and 10 57 mg ( Sargassum thumbergii ) per gra m. The percentage of essent ial amino acids were between 45 22 (Ulva lactuca ) and 28 28 ( Enteromorpa sp.). This method is rapid, sensitive, accurat e and reproducible efficient and precise reliable. The results provide reference for exploitation of seaweeds resources.