Cajal间质细胞在先天性巨结肠乳鼠模型不同肠段的分布变化研究

Distribution of interstitial cells of Cajal in different segments of aganglionosis Hirschsprung's disease neonatal rat model.

【目的】建立乳鼠巨结肠模型,观察其结肠不同肠段中Cajal间质细胞(interstitial cells of Cajal,ICC)分布变化,探讨ICC在先天性巨结肠(Hirschsprung's disease,HD)发病中的作用。【方法】用0.5%苯扎氯铵(benzalkonium chloride,BAC)处理6～7日龄的SD乳鼠降结肠15 min的方法建立先天性巨结肠乳鼠模型,免疫荧光检测ICC在乳鼠巨结肠模型不同肠段的分布变化。【结果】术后4周通过大体观察、组织病理学、免疫组织化学鉴定先天性巨结肠乳鼠模型建立成功。免疫荧光检测ICC在不同肠段的分布。正常组结肠组织ICC主要分布于粘膜下丛和肌间丛,ICC呈连续性分布,相互连接形成网络状结构。狭窄段结肠组织ICC的分布显著减少或消失,较移行段、扩张段和对照组差异均有统计学意义(P<0.01),ICC的网络状结构完全破坏,残存ICC形态异常。移行段结肠组织内ICC的分布较对照组和扩张段组均减少(P<0.05),其形态部分接近正常,但ICC缺乏连续性分布,未能形成正常的网络状结构。扩张段与对照组结肠组织内ICC分布,差异无统计学意义(P>0.05)。【结论】先天性巨结肠乳鼠模型不同肠段ICC的数量、形态、分布不同。狭窄段ICC形态变钝、突起减少或消失。ICC分布减少、形态异常可能是巨结肠发生发展的原因之一。

[Objective] To establish Hirschsprung＇s disease（HD） neonatal rat model and observed the distribution of interstitial cells of Cajal（ICC）in different segments of Hirschsprung＇s disease neonatal rat model, explore ICC in Hirschs prung＇s disease pathogenesis. [Methods] neonatal rats,6-7 days old, 0.5% benzalkonium chloride （BAC） solution was applied to the descending colon for 15 minutes to set up this animal model. The distribution of ICC in different segments of Hirschsprunges disease neonatal rat model were detected by immunofluorescence. [Results] 4 weeks after operation by general observation, HE staining, immunohistochemical staining identified Hirschsprung＇s disease neonatal rat model was successfully created. The distribution of ICC in colon were detected by immunofluorescence. ICC were mainly distributed in submucosal plexus and myenteric plexus of the distal colon. ICC were distributed continuously and formed a network. In the segments of aganglionosis rat, compared with control group.the ganglionic segments group and, ICC were decreased obviously in aganglionic segments group （P〈0.01）. The ICC network were disappeared, and the configuration of the residual ICC were abnormal. In the transitional zone group, ICC were reduced than those of ganglionic segments group and control group （P〈0.05）, Their configuration were closed to normal, but the ICC did not form a normal network. There were no difference of the distribution of ICC in ganglionic segments group and control group（P〉0.05）. [Conclusions] The hum ber, morphous and the distribution of ICC are different in each segments of Hirschsprung＇s disease neonatal rat model. In most aganglionic segments, ICC is absence, the relic ICC gets blunting, decreased or disappeared foot process. The abnormal distribution, number and morphous may be part of the cause to in the development of HD.