应用快速培养法与real-time PCR检测儿童肺炎支原体

Evaluation of the clinical utility of Mycoplasma pneumoniae PCR test on a respiratory sample compared to culture in the diagnosis of Mycoplasma pneumonia.

【目的】探讨肺炎支原体快速培养与实时荧光定量PCR在儿童支原体感染早期快速诊断中的价值,寻求更适合临床应用的肺炎支原体检测方法。【方法】对106例呼吸道感染儿童患者咽拭子标本,同时应用肺炎支原体快速鉴定培养法进行MP培养检测和实时荧光定量PCR检测MP-DNA,并分析两种方法结果。【结果】106例呼吸道感染患儿咽拭子标本中肺炎支原体快速培养阳性31例,检出率是29.3%(31/106),PCR方法阳性28例,检出率是26.4%(28/106)。两种方法检出阳性病例不完全相符。联合检查检出率为46.2%。【结论】两种检测方法各有优、缺点,联合应用可以提高检出率,减少漏诊。

[Objective] To approach a suitable detection method of mycoplasma pneumornia through study of realtime PCR and fast culture. [Method] 106 children due to respiratory infection were applied real-time PCR and mycoplasma pneumornia cultivation at the same time, compared results and analyzed forte and demerit. [Results] Detection rate of mycoplasma pneumornia was 29.3% and 26.4% in cultivation and PCR respectively. All the two results were not completely match. Detection rate of combination was 46.2%. [Conclusions] Anyone of two methods has its own merit and default. Combination of PCR and culture may provide diagnostic information on the aetiology of mycoplasma pneumoniae in- fections.