朝鲜蓟叶中绿原酸提取技术的研究

Study on Extraction Technique of Chlorogenic Acid from Artichoke Leaves

【研究目的】研究采用朝鲜蓟新鲜叶片和干燥叶片进行绿原酸提取的最佳提取方式。【方法】采用不同浓度的盐酸水溶液、乙醇、微波及高温处理等几种方式处理鲜叶样品;采用乙醇和盐酸水溶液处理干燥叶片,比较不同方式下绿原酸的提取效果。【结果】采用0.1mol/L盐酸水溶液和漂烫90s后水提取2种方法对鲜叶中绿原酸的提取效果最好。干叶中绿原酸的提取效果以70%乙醇作为提取剂的提取率最高。【结论】采用适宜的提取条件分别从朝鲜蓟鲜叶和干叶中提取绿原酸切实可行。

[OBJECTIVE]To study the appropriate extraction method of chlorogenic acid from fresh and dry leaves of artichoke.[METHODS]Using different concentration of hydrochloric acid,ethanol,microwave treatment and heat-treatment to extract chlorogenic acid from fresh leaves; different concentration of hydrochloric acid,ethanol were used to extract chlorogenic acid from dry leaves. The extraction rate was compared with those different extraction methods.[RESULTS]Extracted with 0.1mol/L HCl and extracted with water after blanching leaves for 90 seconds showed highest extract efficient for fresh leaves. While 70% ethanol as the solvent showed high extraction rate for dry leaves of artichoke.[CONCLUSION]It was practicable to adopt the appropriate chlorogenic acid extraction methods from fresh and dry leaves of artichoke.