通过玉米茎尖转化耐草甘膦基因2mG2-epsps及抗性鉴定

Transformation of Tolerant-gyphosate 2mG2-epsps Gene by Maize Shoot Tip and Identification of Resistance

以X2211、78599等10个优良玉米自交系的茎尖为受体材料,采用农杆菌介导和真空负压法将抗除草剂基因2mG2-epsps转入玉米茎尖生长点分生组织细胞,转化后的茎尖经共培养3 d,植入花盆,3叶1心经除草剂（glyphosate,草甘膦）筛选,产生耐草甘膦1‰~1.25‰植株,移栽大田。从种子萌发准备茎尖到转化苗喷施除草剂筛选、移栽大田,整个周期只需30 d左右。同时,经特异PCR检测表明：2mG2-epsps基因已整合进部分植株。

10 elite maize inbred lines , such as X2211 and 78599, their shoot tip meristematic cell were thansfered via Agrobacterium with plasmid construct that contained 2mG2-epsps gene under vacuum negative pressure. The transgenic plants were co-cultured for 3 days,then planted in flowerpot,selected by glyphosate at 3 leaves and I heart-leaf per/od and tolerant-glyphosate （ 1‰ - 1.25 ‰） plants were and then, planted out to field. From seed germination to tolerant-plant planted out, the whole time was about 30 days. At the same time, the allelespecific PCR analysis demonstrated that the 2mG2-epsps gene was transformed into the maize genome in some transgenic plants.