杨梅Cu/Zn超氧化物歧化酶基因(MrSOD1)cDNA的克隆及表达分析

Molecular Cloning and Expression Analysis of Cu/Zn Superoxide Dismutase Gene(MrSOD1) cDNA from Myrica rubra

以杨梅叶为材料,利用RT-PCR技术克隆Cu/Zn超氧化物歧化酶基因,并利用半定量RT-PCR方法分析该基因在不同组织中的表达。获得1个杨梅Cu/ZnSOD基因编码区全长cDNA序列,长度为461bp,编码一个由152个氨基酸残基组成的蛋白质。该蛋白质具有Cu/ZnSOD的特征信号序列;序列同源性分析表明,其与GenBank中注册的Cu/ZnSOD序列的同源性均在77%以上。该基因命名为MrSOD1(GenBank登录号:GQ404510)。半定量RT-PCR分析显示,MrSOD1在4种组织中表达量为:果>叶>花>枝条。本试验为进一步研究MrSOD1的时空表达特性、调控途径及杨梅抗氧化伤害的分子机理奠定基础。

RT-PCR technology,and the expression levels of this gene in different tissues were found through semi-quantitative RT-PCR method.The full-length was 461 bp encoding a 152 amino acids Cu/Zn-SOD ortholog.The sequences contained the Cu/Zn-SOD family signature sequence and had the homology more than 77% comparing with other Cu/Zn-SOD from GenBank.This gene was named as MrSOD1 （GenBank accession No.GQ404510）.The expression levels of MrSOD1 in different tissues were fruitleafflower shoot.The study provided a way for deep research on the expression and regulation of MrSOD1,and the molecular mechanism of anti-oxidative effect in Myrica rubra.