Ni-SOD模拟物的合成及其SOD活性测定

Synthesis and SOD-like Activity of Ni-superoxide Dismutase Mimics

为研究Ni-SOD活性中心的结构与清除超氧阴离子自由基活性的关系,合成了3个以Ni2+为中心离子的SOD模拟物:Ni(bim)2(gly)(21),Ni(bim)3(L-asp)(2)和Ni(bim)3(L-glu)(3),用元素分析、摩尔电导率、红外光谱和紫外-可见光谱对其进行了表征。此外,用氮蓝四唑(NBT)光还原法测定了模拟物1—3的SOD活性,其IC50值分别为0.25、0.25和0.38μmol/L。结果表明:3个模拟物均具有相对较高的催化歧化超氧阴离子自由基O2·-的活性。这类SOD模拟物在植物抗逆增产方面有潜在的应用价值。

To get the relationship between central structure of Ni-superoxide dismutase and scavenging activity to superoxide anion,three Ni-SOD mimics,Ni（bim） 2 （gly） 2 （1）,Ni（bim） 3 （L-asp） （2） and Ni（bim） 3 （L-glu） （3） were synthesized and characterized by element analysis,molar conductivity,infrared absorption spectrum and electronic absorption spectrum.The SOD-like activities of the mimics above have been determined by nitroblue tetragolium （NBT）-illumination method,and the IC 50 values of mimics 1-3 are 0.25,0.25 and 0.38 μ mol/L,respectively.It shows that the complexes possess high SOD-like activity.The result indicates that the SOD mimics will be used as the regulators of plant ＇ s growth against the adversity stresses.