不同磷钙元素水平番茄营养液中细菌多样性

Bacterial Diversity in Tomato Hydroponic Culture Solution with Different Element Ca and P Level

为研究不同磷钙元素水平番茄营养液中的细菌多样性,采用从营养液中直接提取微生物总DNA,并用细菌16SrDNA特异性引物进行PCR扩增、变性梯度凝胶电泳(DGGE)、克隆和测序等一系列分子生物学手段。结果表明,各种元素水平的番茄营养液,均有相似的条带,只是亮度上有些差异。与对照相比,各处理元素水平营养液中的细菌DGGE条带均有变化。对照DGGE的条带分布相对均匀,而其他样品的DGGE条带分布多不均匀。番茄营养液中的细菌类群主要包括:Proteobacteria(Alpha,Beta,Gamma和Unclassified),Bacteroidetes和Gram Positive Bacteria。磷钙元素水平对番茄营养液中的细菌多样性具有一定的影响。

To study the bacterial diversity in tomato hydroponic culture with different element Ca and P level.Using DNA directly extracted,bacterial 16S rDNA PCR amplification,DGGE,cloning and sequencing methods.The results showed that each hydroponic culture of element level had the similar DGGE bands patterns.Compared with the control,every DGGE band patterns of the hydroponic culture changed differently.The bands distribution in the control was more evenly than that of the treatments.The main bacterial divisons in tomato hydroponic culture were Proteobacteria （Alpha,Beta,Gamma and Unclassified）,Bacteroidetes and Gram Positive Bacteria.The bacterial diversity in tomato hydroponic culture has changed with the element Ca and P level.