江苏省两个日本沼虾群体的遗传多样性ISSR分析

ISSR Analysis of Genetic Diversity of Two Populations of Macrobrachium nipponense in Jiangsu Province

利用ISSR分子标记技术对江苏长江和太湖2个地区青虾(Macrobrachium nipponense)群体的遗传多样性进行了分析。结果显示,77个ISSR引物中有6个引物能扩增出清晰条带,6个引物对2个群体各24个样品扩增出44个位点。青虾长江和太湖两群体的多态位点比率均为63.64%,Shannon's指数分别为0.348 0、0.346 5。Shannon's指数估算和AMOVA分析均显示日本沼虾的遗传变异主要来自于群体内个体间。日本沼虾UPGMA系统树表明:两个地区日本沼虾群体的遗传多样性均处于较高水平;日本沼虾江苏长江和太湖两个群体间存在一定的遗传分化。

Inter-simple sequence repeat （ISSR） was used to detect the genetic variation of two geographical populations of Macrobrachium nipponense. The individuals of M. nipponense were collected from the Changjiang River and Taihu Lake. Six primers, which were sifted from all 77 ISSR primers, produced 44 polymorphic markers from two populations. The proportions of polymorphic loci of Changjiang and Taihu populations were all 63.44%, and Shannon＇s Information indexes were respectively 0. 348 0 and 0. 346 5. These results showed that the genetic diversity indexes of the two populations were at the same level. Shannon＇s Information index and AMOVA analysis indicated that genetic variation of M. nipponense was derived mainly from individuals within populations and had no significant genetic divergence between populations. The UPGMA tree based on ISSR data supported this result and no distinct variation was detected. The results of ISSR analysis indicated that two populations of M. nipponense presented lower genetic diversity; and there was a relatively normal level of genetic variation and some significant genetic differentiation between two populations.