常压低温等离子体对小鼠创伤性皮肤溃疡愈合的影响

Effect of cold atmospheric plasma on skin ulcer healing

目的 探讨常压低温等离子体（CAP）对Balb/c小鼠创伤性皮肤溃疡愈合的影响.方法 在150只Balb/c小鼠背部两侧各造一直径6 mm的溃疡模型,将其分为对照组（溃疡自然愈合）、He-Ne激光组（溃疡接受He-Ne激光治疗）及CAP组（溃疡接受CAP治疗）,每组50只.术后第4,7,10,14天计算各组创面愈合面积百分比;3组各处死10只小鼠,两侧溃疡均被切取进行病检,按同一评分系统评分.术后第7天,以免疫组化技术检测溃疡组织中血管内皮生长因子（VEGF）的表达,图像分析系统定量.结果 与对照组比较,CAP组在术后第7,10天,平均创面愈合面积百分比明显增大（P＜0.01）;在术后第7,10,14天,平均病理评分也明显提高（P＜0.05,P＜0.01,P＜0.05）.VEGF的表达水平,CAP组较对照组、He-Ne激光组明显提高（P＜0.01）.结论 CAP具有促进创伤性皮肤溃疡愈合的作用,该作用可能与其提升VEGF的水平有关.

Objective To investigate the effect of cold atmospheric plasma （CAP） on the healing of skin ulcers using Balb/c mice. Methods Wounds with a diameter 6 mm were created on each side of the backs of BalB/c mice （ n = 150） using a punch bioptome. The mice were assigned randomly into a control group （ wounds healed naturally）, a laser group （wounds treated with a He-Ne laser for 10 min daily） and a CAP group （wounds treated with CAP for 10 min daily）. Wound healing was evaluated on postoperative days （PODs） 4, 7, 10 and 14 in terms of percent wound closure. Ten mice per group were sacrificed on each of the evaluation days. Both wounds were removed and a histological examination was conducted. A scoring system was used to evaluate the wounds. The expression of vascular endothelial growth factors （VEGFs） in the wounded tissue was detected by using immunohistochemical methods on POD 7. The results were quantified using an HPIAS-1000 system. Results Compared with the control group, the average percentage of wound healing was significantly greater in the CAP group on PODs 7 and 10. The average scores on the histological examination were significantly higher in the CAP group on PODs 7, 10 and 14. Compared with the other two groups, the expression of VEGF was up-regulated significantly in the CAP group.Conclusions CAP can positively affect the wound healing process. This might be related to the up-regulation of VEGF in the wounded tissues.