隐性TGF-β结合蛋白-3在小鼠下颌第一磨牙牙囊中的表达及意义

Gene expression of latent TGF-β binding protein-3 in the follicle of mouse first mandibular molar in mice

目的:研究小鼠牙萌出过程中隐性TGF-β结合蛋白-3(LTBP-3)在下颌第一磨牙牙囊中的表达情况,探讨其在牙萌出过程中的作用。方法:取不同天数的新生昆明小鼠下颌第一磨牙牙胚,剥离牙胚外层的牙囊组织,采用反转录-聚合酶链式反应(RT-PCR)技术,检测牙囊中LTBP-3mRNA的表达,应用凝胶成像系统定量软件和SPSS13.0软件包,通过单因素方差分析,比较基因表达的差异。结果:LTBP-3mRNA在牙囊组织中有表达,且表达随牙胚发育的时期不同而变化:随鼠龄增长,在牙囊中呈递增表达,其中出生后第9.5天和第7.5天表达量高于出生后第1.5天(P分别<0.05和<0.01),出生后第9.5天表达量高于第3.5天(P<0.05),其余时间点间的表达水平无统计学差异。结论:LTBP-3参与了牙囊分化过程的调节,其mRNA在小鼠下颌第一磨牙牙囊中于出生后第1.5天至第9.5天呈递增表达,提示可能在小鼠牙萌出中发挥作用。

PURPOSE：To observe and analyze the gene expression of latent TGF-β binding protein-3（LTBP-3）in mice first mandibular molars during tooth eruption period.METHODS：The first mandibular molar germs of KM mice, from postnatal 1.5 to 9.5 days, were dissected.Anatomical microscope was used to observe and separate dental follicles of each time-point.After total RNA extracted from separated dental follicles of the first mandibular molars, reverse transcription-polymerase chain reaction （RT-PCR） was performed using LTBP-3 specific primers to detect its gene expression.Then densitometry analysis was performed using Gene Genius Bio-Imaging system and GeneSnap software.Expression differences between any two time points were assessed by LSD test in one-way ANOVA at α=0.05 level using SPSS 13.0 software package.RESULTS：A circle of fibrae sac like loose tissue could be seen around all the germs in anatomical microscope and could be carefully dissected from the germs.There was an aging progressive gene expression of LTBP-3 in the dental follicles of mice first mandibular molars among the detecting time-points.LSD test analysis revealed that the expression of both P7.5 and P9.5 was higher than that on the P1.5 （P〈0.05 and P〈0.01, respectively） and P9.5 expression level was higher than that on the P3.5 （P〈0.05）, while there was no difference in expression between any other two detecting days.CONCLUSIONS：LTBP-3 is confined to have an aging progressive gene expression in the follicles of mice first mandibular molars during tooth eruption period.LTBP-3 may play a role in the events of mice teeth eruption.