云南洱源牛街热泉原核微生物多样性分析

Prokaryotic microbial phylogenetic diversity of "Eryuan Niujie" hot spring in Yunnan province,China

【目的】通过分析富含高分子有机物的云南洱源牛街热泉原核微生物16SrRNA基因克隆文库,丰富对高温热泉原核微生物多样性的认识,为进一步开发和利用该热泉微生物资源奠定基础。【方法】构建洱源牛街高温热泉原核微生物16SrRNA基因克隆文库,通过测序和序列相似性比对以及聚类分析研究该热泉原核微生物的多样性。【结果】该热泉原核微生物以细菌为主,包括变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、梭杆菌门(Fusobacteria)等在内的约10个细菌类群,其中变形菌门中的β-变形菌纲(β-Proteobacteria)为优势菌群,其次为拟杆菌门(Bacteroidetes)、绿菌门(Chlorobi);古菌的生物量和丰度较细菌少,分属广古菌(Euryarchaeota)和泉古菌(Crenarchaeota)两个类群,以广古菌为优势类群。

[Objective]We analyzed the prokaryotic microbial diversity of Eryuan Niujie hot spring,in Yunnan Province,to enrich our knowledge about thermo-stable microbes. [Method]We constructed bacterial and archaeal 16S rRNA gene libraries,analyzed the sequences and constructed phylogenetic trees to learn the prokaryotic microbial diversity. [Result] The majority of the prokaryotic microbes in this hot spring were bacteria,while β-Proteobacteria was the most abundant,next were Bacteroidetes and Chlorobi; the abundance and diversity of archaea were both less than that of bacteria,including Euryarchaeota and Crenarchaeota,while Euryarchaeota was the most abundant.