Toll样受体2和4在麻风患者皮损中的表达

Expression of Toll-like Receptor 2 and 4 in Lesions of Leprosy

目的观察Tol1样受体(TLR)2和4在麻风患者皮损中的表达情况,探讨它们与麻风发病的相关性。方法选择20例结核样型麻风(TT)、20例瘤型麻风(LL)及20例正常人的皮肤标本,用免疫组化法研究TLR2和TLR4的表达;采用图像分析系统测定组织切片中TLR2和TLR4阳性表达的积分光密度值,并应用SPSS13.0统计软件进行统计学分析。结果 20例TT和20例LL皮损的表皮和真皮均可见明显的TLR2表达;而TLR4仅在表皮基底细胞层呈弱表达,真皮内未见表达。20例正常人皮肤表皮均有较弱的TLR2表达,而无TLR4表达,真皮未见TLR2及TLR4表达。三组间TLR2和TLR4阳性表达总体比较,差异有统计学意义(P<0.01);两两比较结果显示:TLR2和TLR4在TT组和LL组表达均强于正常对照组(P均<0.01),TLR2和TLR4在TT组中的表达均强于LL组(P均<0.01)。结论 TLR2和TLR4在麻风患者的皮损中表达增强,提示它们作为模式识别受体可能介导了麻风的天然免疫反应,参与了麻风的感染与免疫过程。

Objective To study the expression of Toll-like receptor 2 （TLR2） and TLR4 in lesions of leprosy.Methods Immunochemistry was employed to study the expression of TLR2 and TLR4 in skin samples from 20 tuberculoid leprosy （TT）,20 lepromatous leprosy（LL） and 20 normal human.The intergral optical density （IOD） of the positive expression of TLR2 and TLR4 was measured with Image-pro plus system and all statistics were analyzed by SPSS 13.0.Results TLR2 was strongly expressed in epidermis and dermis of all the 20 specimens of TT and LL.The weak expression of TLR2 was found only in the epidermis of normal skin.TLR4 was weakly expressed in stratum basal on both TT and LL,no expression in dermis.The positive expression of TLR2 and TLR4 among the TT,LL and control were significant difference （P〈0.01）.The expression of TLR2 and TLR4 in both TT and LL was stonger than that of in control （P〈0.01）.The expression of TLR2 and TLR4 in TT was stonger than that of LL （P〈0.01）.Conclusions The expression of TLR2 and TLR4 in the skin lesions of patients with leprosy was significant increased.It was suspected that TLR2 and TLR4 may mediate and take part in the innate immune response of leprosy as a pattern recognition receptors.