发育后期牙胚的体外培养

In vitro culture of tooth germ in late development stage

目的:观察大鼠发育后期牙胚在体外器官培养条件下的生长情况。方法:分离出生后7～8d的大鼠磨牙完整牙胚,进行体外器官培养,组织学观察牙胚发育情况。结果:体外培养的前12d,牙胚维持了原本的形态和结构,但没有进一步发育,随后牙胚发生萎缩。结论:发育后期的牙胚可在体外进行短期培养,但不能继续发育。

AIM：To observe the growth of rat molar germs in late development stage under the condition of organ culture in vitro.METHODS：Intact molar germs of 7 days postnatal rats were isolated,cultured in vitro and observed by histological methods.RESULTS：In the first 12 days of organ culture in vitro,rat molar germ maintained the original form and structure without further development.Afterwards,atrophy of the tooth germs was observed.CONCLUSION：Tooth germ in late development stage can be cultured in vitro for a short time,but can not continue to develop.