摘要
高尔基体作为主要的分泌细胞器,其功能复杂。从细胞内分离出纯度较高的高尔基体有利于对其功能的深入研究。通过胃癌细胞SGC7901的大量体外培养,差速离心结合蔗糖密度梯度离心法纯化分离出高尔基体后,电镜及中性红超活染色进行形态学鉴定,高尔基体标志酶半乳糖基转移酶Galse及硫胺素焦磷酸酶TPPase活性测定进行纯度分析,首次从胃癌细胞SGC7901内成功分离出纯度较高的高尔基体,为对高尔基体功能的深入研究奠定基础。
The Golgi complex is the central organelle of the secretory pathway and has many complicate functions.The endeavours to isolate and purify the Golgi apparatus from cultured cells will benefit further investigation of Golgi.A large number of gastric cancer cells SGC7901 were cultivated in vitro,then Golgi apparatus were isolated from the cells by differential centrifugation combined with sucrose density gradient ultra-centrifugation.Its purity was characterized biochemically by enzymatic assays,morphologically by electron microscopy（EM） and neutral red supravital staining.Finally the Golgi complex was successfully fractionated from gastric cancer cells SGC7901.The first successful isolation of Golgi apparatus from gastric cancer cells SGC7901 by using ultra-centrifugation will lead to research into the function of Golgi apparatus.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2010年第5期1085-1088,共4页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(30672431)
教育部博士点基金资助项目(20060631006)
关键词
高尔基体
胃癌细胞
亚细胞器分离
蔗糖密度梯度离心
Golgi apparatus； Gastric cancer cell； Subcellular separation； Sucrose density gradient ultra-centrifugation；
