基因HLA-A0205、HLA-A30与成人迟发自身免疫性糖尿病的关系研究

Association of HLA-A0205 and HLA-A30 with latent Autoimmune Diabetes in Adults

研究中国成都地区汉族成人迟发自身免疫性糖尿病(LADA)患者中人类白细胞抗原(HLA)A0205、A30基因频率分布,探讨它们与LADA的关系。选取成都地区符合纳入标准的LADA组患者41例(男25例,女16例,年龄52.17±11.56岁),2型糖尿病(T2DM)组患者40例(男26例,女14例,年龄60.69±12.20岁),正常对照(NC)组40例(男15例,女25例,年龄44.69±13.29岁)。应用巢式聚合酶链反应-序列特异引物(nested PCR-SSP)结合测序技术,采用2检验(双侧α=0.05),对三组研究对象分别比较了HLA-A0205、HLA-A30基因频率分布。121例DM及正常对照中,检出HLA-A0205 2例,LADA组及NC组各1例,等位基因频率分别占2.44%和2.5%;HLA-A30共5例,LADA组2例,T2DM组2例,NC组1例,等位基因频率分别占4.87%、5.0%和2.5%。均无统计学意义差异(P>0.05)。提示HLA-A0205、HLA-A30在中国成都地区汉族人中分布频率低,各组间分布差异无统计学意义。尚不能说明HLA-A0205、HLA-A30与中国成都地区汉族LADA有关。本研究样本较少,这一结论尚需进一步扩大样本,最好行家系连锁分析验证。

To investigate the association of HLA-A0205 and HLA-A30 with latent autoimmune diabetes mellius in adults（LADA） in Chengdu Hans,121 subjects（41 cases of LADA,40 cases of T2DM,and 40 normal controls） were enrolled in the study.The frequencies of HLA-A0205 and HLA-A30 were determined by nested PCR-SSP and direct sequencing,respectively.The allele frequencies of patient groups and of normal controls were compared by chi-square test using SPSS 11.0（α=0.05）.Hardy-Weinberg equilibrium was tested with use of software HWE（α=0.05）.Data from the subjects showed： HLA-A0205 was present in 1 patient with LADA and in 1 normal control（2.44% and 2.5%,respectively）,HLA-A30 was present in 2 patients with LADA,in 2 patients with T2DM and in 1 normal control（4.87%,5.0% and 2.5%,respectively）.There was no statistically significant difference between the allele frequencies of the three groups.These results suggest that HLA-A0205,HLA-A30 may not be related to LADA in Chengdu Hans.Yet,further studies with larger sample size may be needed to warrant this conclusion.