2825例皮肤病患者皮损直接免疫荧光检查结果分析

Anaysis of Results of Skin Lesion Test Using Direct Immunofluorescence Technique on 2 825 Patients with Dermatosis.

目的为了探讨直接免疫荧光检查对某些皮肤病的意义。方法采用抗人Ｉｇ（ＩｇＧ、ＩｇＡ、ＩｇＭ和补体Ｃ３）直接免疫荧光技术对２８２５例（８９个病种）皮肤病患者皮损作了检查。结果９２６例系统性红斑狼疮皮损区基底膜阳性占９５％，曝光区正常皮肤基底膜阳性占８０％，而２８７例盘状红斑狼疮皮损基底膜阳性占９２％，曝光区正常皮肤均阴性；６２例混合结缔组织病皮损区阳性为８５％；１８４例皮肌炎皮损血管壁阳性７０％；７３例系统性硬皮病皮损区阳性７０％；２１１例寻常型银屑病皮损区角质层阳性９３％；１２５例寻常性天疱疮皮损区表皮细胞间阳性９５％；８５例大疱性类天疱疮的阳性为９２％；７１例疱疹样皮炎的阳性为９０％；１１６例血管炎病灶区血管壁阳性为９０％。结论提示ＳＬＥ、ＤＬＥ及大疱性皮肤病皮损的免疫荧光标记形态具有特征性改变。而且，直接免疫荧光检查对其它皮肤病的诊断与鉴别诊断也有重要意义。

Objective In order to explore the meanings of direct immunofluorescence test for some dermatoses.Method We tested 2 825 cases of patients(including 89 dermatoses) by direct immunofluorescence technology(DIF) of goat anti human Ig,which was included IgG,IgA,IgM and C 3 complement.Results 95% positive base membrane Ig in skin lesion areas of 926 patients with Systemic Lupus Erythematosue(SLE),80% in normal skin sun exposure areas,92% base membrane Ig positive in skin lesions of 287 patients with DLE,negative in all normal skin sun exporure areas.85% in skin lesions of 62 patints with MCTD,70% in blood vessel wall of 184 cases of dermatomyositis,70% in skin lesions of 73 patints with systemic scleroderma,93% in skin lesion keratodermas of 211 patinets with psoriasis,95% in skin lesion epidermis of 125 patients with vulgaris pemphigus;92% in 85 cases of bullous pemphigoid;90% in 71 patients with dermatitis herpeti formis;90% in bllood vessal wall of lesions of 116 patients with vasculitis,respectivly.Conclusion It was suggested that SLE,DLE and vulgaris pemphigus had morphologically characteristics of DIF,furthermore,DIF technique is important for diagnosis and differential diagnosis of the other dermatosis.