黄瓜单性花控制基因M的结构分析

Characterization Analysis of the Monoecious-Determine M Gene in Cucumber (Cucumis sativus L.)

黄瓜是研究植物性型决定机制的模式植物。本研究主要从基因序列分析比对,启动子序列分析,系统进化分析和蛋白质保守结构域预测等方面研究黄瓜单性花控制基因M(CsACS2)的结构特点,结果表明:a.黄瓜CsACS2基因与甜瓜CmACS7基因可能为直源基因;b.CsACS2基因启动子中含有乙烯响应元件(ERE元件)和花器官发育相关元件(LEAFY元件);c.CsACS2基因所编码蛋白与拟南芥中AtACS7基因编码蛋白进化关系较近,同源性最高达72.8%;d.该基因编码的ACC合成酶中存在一个潜在的保守结构域S-YF-GW。这些分析结果有助于更深入地研究黄瓜单性花控制基因CsACS2的特性和潜在的生物学作用。

Cucumber （Cucumis sativus L.） is becoming a model to study the sex determination mechanism in plant. The monoecious- determine M gene had been cloned as a key enzyme （CsA CS2） which takes part in the plant hormone ethylene biosynthesis. In this study, we use the amino acid alignment, cis element analysis in promoter sequence, phylogenetic analysis and conserved domain prediction to study the CsA CS2, and the results showed that： a. cucumber CsA CS2 may be ortholog gene with melon CmACST; b. two ethylene-response elements （ERE motif） and one floral organ development relative element （LEAFY motif） are found in the promoter of CsA CS2 ; c. CsA CS2 has a high homology （72.8%） with A tA CS7 in A rabidopsis; d. a putative conserved motif. （S-YF-GW） has been found in this ACC synthase. These results are useful to reveal the characteristics and potential biological role of the monoecious-determine gene CsA CS2 in cucumber.