哲罗鲑雌性蛋白的研究

Female-related protein in taimen Hucho taimen

采用柱层析、蛋白质免疫印迹和电泳等方法,对哲罗鲑Hucho taimen的雌性蛋白进行了系统研究。结果表明:哲罗鲑血清和卵黄中均存在雌性蛋白,其雌性蛋白的性质相同,均为糖脂磷蛋白;与血清雌性特异蛋白一样,卵黄雌性特异蛋白的相对分子质量也为460 000,由相对分子质量分别为137 800、84 200和10 800的3个亚基组成,等电点为5.60;卵黄雌性蛋白的兔抗血清均与哲罗鲑雌性血清发生免疫反应,不与雄性血清发生免疫反应,表明这两种蛋白均具有雌性特异性;两种蛋白的抗血清可以相互检测,表明哲罗鲑雌鱼血清中雌性蛋白与卵黄中的雌性蛋白在免疫原性上和结构上具有较高的同源性。

Female-related protein was purified from serum and egg yolk in broodstock taimen Hucho taimen by chromatography and electrophoresis,and the chemical properties were analyzed.The results showed that the FYSP protein was observed in the second peak of the crude extract in the eggs,with identical characters with female special serum protein existed in the female serum.SDS-PAGE revealed that the two kinds of FYSP with molecular weights of 137 800,84 200 and 10 800 appeared as three bands.These proteins were stained by red oil O,Molecular Proes＇ Pro-Q Emerald 300 Glycoprotein Gel stain and Pro-Q Diamond Phosphoprotein Gel Stain,indicating that they are phospholipins-glycoprotein.The PI of FYSP in egg yolk was 5.60.Western-blotting and immunodiffusion indicated that antiserum against FYSP cross-reacted with the female serum,but not cross-reacted with the male serum.