摘要
研究了应用DNA测序峰形图定量和精细研究碱基变异分析的方法,根据碱基变异峰形图的面积比值,得出变异比例大小。采用相关Windows API函数获取碱基变异点处的DNA峰形图,对其进行清理多余线条及修补线条等处理,计算出不同峰形图的面积比值,并给出了软件的关键设计。回收实验结果以及临床数据表明,该软件采用的设计思想正确,检测准确度高,有良好的应用前景。
A quantitative analysis ofgene mutation by DNA sequencing peak shape is explored, with mutation percentage obtained based on area ratio of gene mutation peak shape. DNA peak shapes at gene mutation points are gained by adopting the related Windows API function. Area ratio of various peak shapes, with extra lines cleared offand some lines repaired, is calculated and the key design of the software is illustrated. Recycle experiment and clinical data revealed that the software, with correct design and high precision, has a sound annlication nrosnect.
出处
《计算机工程与设计》
CSCD
北大核心
2010年第21期4724-4727,共4页
Computer Engineering and Design
基金
国家自然科学基金项目(30671872
30771899)
南方医院新技术基金项目(2007005)
